By Peter Hahn, Elizabeth Scanlan (auth.), Wolfgang Bielke, Christoph Erbacher (eds.)

Gene supply into Mammalian Cells: an summary on present methods hired In Vitro and In Vivo, by means of Peter Hahn and Elizabeth Scanlan

Strategies for the practise of artificial Transfection Vectors, via Asier Unciti-Broceta, Matthew N. Bacon, and Mark Bradley
Cationic Lipids: Molecular Structure/Transfection task Relationships and Interactions with Biomembranes, through Rumiana Koynova and Boris Tenchov
Hyperbranched Polyamines for Transfection, through Wiebke Fischer, Marcelo Calderon, and Rainer Haag

Carbohydrate Polymers for Nonviral Nucleic Acid supply, via Antons Sizovs, Patrick M. McLendon, Sathya Srinivasachari,

and Theresa M. Reineke
Cationic Liposome–Nucleic Acid Complexes for Gene supply and Silencing: Pathways and Mechanisms for Plasmid DNA and siRNA, by way of Kai ok. Ewert, Alexandra Zidovska, Ayesha Ahmad, Nathan F. Bouxsein, Heather M. Evans, Christopher S. McAllister, Charles E. Samuel, and Cyrus R. Safinya
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Partially-convergent strategies have been applied for the synthesis of unsymmetrical PAMAM dendrimers by reaction of divergently-synthesised PAMAM dendrons either via a 1,3-dipolar cycloaddition reaction [205, 206] or through a poly(ethyleneglycol) (PEG) core [207]. In particular the PAMAM–PEG–PAMAM dendrimer showed high transfection efficiency, probably due to enhanced branch flexibility and DNA complexation [207]. 0 Fig. 21 Synthesis of poly(L-lysine) dendrimers been used for the preparation of PAMAM dendrimers [208], this has not been employed in synthesising PAMAM-based transfection vectors.

5a) or synthesising the cationic lipid domain first and then linking two of these through a spacer at the end of the synthetic route [111] (see Fig. 5b). Aryl groups have been used as rigid spacers between the lipophilic groups and the cationic moieties [84, 111, 112] (see Fig. 5b). g. pyridine, imidazole, quinoline) have additionally been employed as headgroups of cationic lipids, in part because charge delocalisation is considered to diminish cellular toxicity [100, 113–125]. Among nitrogen-based heterocycles, quaternary pyridinium salts are the most widely used for the preparation of cationic lipids [118–125], being synthesised by quaternisation of the nitrogen atom [118–122] (see Fig.

23). PAMAM dendrons were assembled by treating deprotected scaffold-bound resin 24 first with an excess of methyl acrylate H N H 2N NH 2 O O Cl3 CH N O O O O O O O HN N O O O O N NO 2 2 2 O 1M NaOH:dioxane O Et3 N, CH 3 CN, 60°C O O N O O O O H 2N N O NH O 2 DIC, HOBt, DCM OH N O NH 2 2 O O O N H (24) Fig. 23 The synthesis of polyamine-bound resin 24 2 NH 2NH 2 H2 O EtOH, reflux O O N O O O O N O OH O N H 40 A. Unciti-Broceta et al. 0 (Base) NH O NH NH2 Fig. 24 Solid-phase synthesis of a G3 PAMAM dendrimer (1,4-addition) and then by reaction of the methyl ester with 1,3-propanediamine (see Fig.

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